import wave
import os
infiles = ["Input1.wav", "Input2.wav"]
outfile = "sounds.wav"
data= []
for infile in infiles:
w = wave.open(os.getcwd()+'/'+infile, 'rb')
data.append([w.getparams(), w.readframes(w.getnframes())])
w.close()
output = wave.open(outfile, 'wb')
output.setparams(data[0][0])
output.writeframes(data[0][1])
output.writeframes(data[1][1])
output.writeframes(data[0][1])
output.close()
파이썬 wav 라이브러리 관련 링크 참조